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KMID : 1004520200360030145
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Journal of Dental Rehabilitation and Applied Science
2020 Volume.36 No. 3 p.145 ~ p.157
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Analysis of cell survival genes in human gingival fibroblasts after sequential release of trichloroacetic acid and epidermal growth factor using the nano-controlled release system
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Cho Joon-Youn
Lee Sung-Bok
Lee Suk-Won
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Abstract
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Purpose: This study was to determine the possible effects of trichloroacetic acid (TCA) and epidermal growth factor (EGF) through cell survival genes of the PI3K-AKT signaling pathway when applying an hydrophobically modified glycol chitosan (HGC)-based nano-controlled release system to human gingival fibroblasts in oral soft tissue regeneration. Materials and
Methods: An HGC-based nano-controlled release system was produced, followed by the loading of TCA and EGF. The
group was divided into control (CON), TCA-loaded nano-controlled release system (EXP1), and the TCA- and EGF- individually loaded nano-controlled release system (EXP2). A total for 29 genes related to the PI3K-AKT signaling pathway were analyzed after 48h of culture in human gingival fibroblasts. Real-time PCR, 1- way ANOVA and multiple regression analysis were performed.
Results: Cell survival genes were significantly upregulated in EXP1 and EXP2. From multiple regression analysis, ITGB1 was determined to be the most influential factor for AKT1 expression.
Conclusion: The application of TCA and EGF through the HGC-based nano-controlled release system can up-regulate the cell survival pathway.
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KEYWORD
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human gingival fibroblast, trichloroacetic acid, epidermal growth factor, PI3K-AKT pathway, cell survival
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